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pcep4 mammalian expression vector  (Thermo Fisher)


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    Thermo Fisher pcep4 mammalian expression vector
    Pcep4 Mammalian Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcep4 mammalian expression vector/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    pcep4 mammalian expression vector - by Bioz Stars, 2026-03
    90/100 stars

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    Photograph of the transfected CHO-s cells obtained with a fluorescence microscope,at magnification of 10X, and the rate of transfection obtained with flow cytometry,24 h after transfection. (A) Untransfected CHO-s cells, (B) CHO-s cells transfected with <t>pCEP4-hFIX</t> wt , (C) CHO-s cells transfected with pCEP4-hFIX K22N , (D) CHO-s cells transfected with pCEP4-hFIX R37N plasmids
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    Photograph of the transfected CHO-s cells obtained with a fluorescence microscope,at magnification of 10X, and the rate of transfection obtained with flow cytometry,24 h after transfection. (A) Untransfected CHO-s cells, (B) CHO-s cells transfected with pCEP4-hFIX wt , (C) CHO-s cells transfected with pCEP4-hFIX K22N , (D) CHO-s cells transfected with pCEP4-hFIX R37N plasmids

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: Studying the Expression Efficiencies of Human Clotting Factor IX Analogs, Rationally-designed for Hyper-glycosylation

    doi: 10.22037/ijpr.2020.112027.13503

    Figure Lengend Snippet: Photograph of the transfected CHO-s cells obtained with a fluorescence microscope,at magnification of 10X, and the rate of transfection obtained with flow cytometry,24 h after transfection. (A) Untransfected CHO-s cells, (B) CHO-s cells transfected with pCEP4-hFIX wt , (C) CHO-s cells transfected with pCEP4-hFIX K22N , (D) CHO-s cells transfected with pCEP4-hFIX R37N plasmids

    Article Snippet: Following Nhe I /Not I digestion, the target fragments, comprising the coding sequences of either the hFIX or its mutants and IRES-EGFP sequence, were subcloned into the pCEP4 expression vector (Thermo Fisher Scientific, USA), downstream to the cytomegalovirus (CMV) immediate early promoter/enhancer to end up with three bicistronic recombinant plasmids namely; pCEP4-hFIX wt , pCEP4-hFIX K22N , and pCEP4-hFIX R37N .

    Techniques: Transfection, Fluorescence, Microscopy, Flow Cytometry